Various clinical presentations have been reported in patients, varying from asymptomatic infections to ulcerative lesions of the skin, which last for many months and may cause disfigurement and stigma. In Tunisia, the disease is distributed mainly in the central and southern parts of the country where it is considered a major public health problem. Zoonotic cutaneous leishmaniasis (ZCL) caused by L. major isolates from patients, insect vectors, and reservoir hosts from different endemic foci should be collected for further analysis. major with similar clinical manifestations occurring within the different geographical regions. Our results revealed a heterogeneous genetic profile for L. AMOVA indicated that the highest variability (99 %) existed within the study regions. ResultsĪnalysis of the genetic diversity among the isolates revealed a high level of polymorphism (43 %) among them. Random amplified polymorphic DNA (RAPD) PCR marker polymorphism was estimated by calculating Nei and Li’s genetic distances and by an analysis of molecular variance (AMOVA). major by restriction fragment length polymorphism typing) from patients experiencing the same clinical manifestations but living in different regions of Tunisia where L.

Our sample comprised 39 isolates (confirmed as L. This work aimed to estimate the genetic variability within different isolates of L.

major strains is available in the literature. major parasites has been suggested as a potential factor influencing their pathogenic variability, little information on genetic polymorphism among L. The clinical course of the disease varies widely among different patients and geographic regions.

Zoonotic cutaneous leishmaniasis caused by Leishmania ( L.) major is endemoepidemic in the Center and South of Tunisia.

Winamova software